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Study of human chromosomes. IV. Labeling of chromosomal proteins with the amino group specific fluorescent reagent fluorescamine

LJ Sciorra, ML Lee and H Wynnyckyj

We describe a method for labeling chromosomal proteins with an amino- group-specific fluorescent reagent, fluorescamine. Chromosomes thus labeled appear either as uniformly fluorescent or as haloes in structure depending on the proteins remaining after treatment with acid- alcohol fixation. Using fluorescamine as a probe, we demonstrate that there is a substantial loss of labeled proteins during the chromosomal preparation and also during the trypsin treatment used in the banding of chromosomes.

Volume 33, Issue 12, pp. 1252-1255, 12/01/1985
Copyright © 1985 by The Histochemical Society


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The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1985