|
|
|
|
 |
|||
|
|||
Localization of eosinophil cationic protein, major basic protein, and eosinophil peroxidase in human eosinophils by immunoelectron microscopic technique
A Egesten, J Alumets, C von Mecklenburg, M Palmegren and I Olsson
An immunoelectron microscopic technique using protein A-gold as a specific marker was used for precise intracellular localization of eosinophil granule proteins. Eosinophils from healthy individuals were isolated in metrizamide gradients. Eosinophil cationic protein (ECP) and eosinophil peroxidase (EPO) were clearly located in the matrix of the large crystalloid-containing granules. In addition, ECP was probably present in the small granules of eosinophils. Major basic protein (MBP) was present in the crystalloid structure of specific granules. This method can be applied in studies of eosinophil degranulation to trace the release of biological effector molecules.
Volume 34,
Issue 11,
pp. 1399-1403,
11/01/1986
Copyright © 1986 by The Histochemical Society
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  R. C. N. Melo, L. A. Spencer, A. M. Dvorak, and P. F. Weller Mechanisms of eosinophil secretion: large vesiculotubular carriers mediate transport and release of granule-derived cytokines and other proteins J. Leukoc. Biol., February 1, 2008; 83(2): 229 - 236. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. R. Eversole, C. D. Mackenzie, and L. J. Beuving A Photoreactive Fluorescent Marker for Identifying Eosinophils and Their Cytoplasmic Granules in Tissues J. Histochem. Cytochem., February 1, 2003; 51(2): 253 - 257. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Daneshpouy, G. Socie, M. Lemann, J. Rivet, E. Gluckman, and A. Janin Activated eosinophils in upper gastrointestinal tract of patients with graft-versus-host disease Blood, April 15, 2002; 99(8): 3033 - 3040. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Karawajczyk, L. Sevéus, R. Garcia, E. Björnsson, C. G. B. Peterson, G. M. Roomans, and P. Venge Piecemeal Degranulation of Peripheral Blood Eosinophils . A Study of Allergic Subjects during and out of the Pollen Season Am. J. Respir. Cell Mol. Biol., October 1, 2000; 23(4): 521 - 529. [Abstract] [Full Text]
|
|
|
|
 |
|
 B. Cai, M. J. Spencer, G. Nakamura, L. Tseng-Ong, and J. G. Tidball Eosinophilia of Dystrophin-Deficient Muscle Is Promoted by Perforin-Mediated Cytotoxicity by T Cell Effectors Am. J. Pathol., May 1, 2000; 156(5): 1789 - 1796. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. A. Giembycz and M. A. Lindsay Pharmacology of the Eosinophil Pharmacol. Rev., June 1, 1999; 51(2): 213 - 340. [Abstract] [Full Text] [PDF]
|
|
| Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact |