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Microimmunoassay permits determination of concentrations in immunohistochemistry controls

DJ Goldstein, MM Davis, RK Naviaux, TL Dailey and TM Ulbright

A competition enzyme-linked immunosorbant assay (CELIA) can be used to determine the amount of antigen needed to immunoabsorb an antiserum. This is demonstrated using both polyclonal and monoclonal antibodies against human amylase. This method is of especial value when the tissue is particularly difficult to obtain, and especially valuable when CELIA has already been completed.

Volume 34, Issue 4, pp. 543-545, 04/01/1986
Copyright © 1986 by The Histochemical Society


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The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1986