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Improved preservation of glycogen in unfixed cyanobacteria embedded at - 82 degrees C in nanoplast

D Frosch, C Westphal and H Bohme

A technique is presented for embedding unfixed cells at -82 degrees C in Nanoplast, a water-soluble melamine resin. By use of this procedure, improved ultrastructural preservation is demonstrated in cyanobacteria. Sections treated in this way are suitable for histochemical techniques such as Thiery's silver staining method for glycogen.

Volume 35, Issue 1, pp. 119-121, 01/01/1987
Copyright © 1987 by The Histochemical Society


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The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1987