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Pyrido[2,1-i]-purines: a new class of fluorescent stains

WM Odijk and GJ Koomen

7-oxo-pyrido[2,1-i]-purine nucleosides 1a and 1b are highly fluorescent molecules with absorptions and emissions in the visible region. They show little or no cytotoxicity; interactions with isolated enzyme systems could not be detected, and these molecules appear to enter the cell via a purine nucleoside-specific carrier. We demonstrate for one case a specific interaction with cell components. The fluorescence of pyrido[2,1-i]-purine 2 is solvent-dependent and stains the lipophilic parts of a cell.

Volume 35, Issue 10, pp. 1161-1163, 10/01/1987
Copyright © 1987 by The Histochemical Society


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