Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by van der Loos, C. M.
Right arrow Articles by Houthoff, H. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by van der Loos, C. M.
Right arrow Articles by Houthoff, H. J.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

An immunoenzyme triple-staining method using both polyclonal and monoclonal antibodies from the same species. Application of combined direct, indirect, and avidin-biotin complex (ABC) technique

CM van der Loos, PK Das and HJ Houthoff

Department of Pathology, Academic Medical Center, Amsterdam, The Netherlands.

For immunohistological analysis, simultaneous detection of multiple cellular epitopes, as compared to single staining of serial sections, is sometimes needed. Therefore, immunoenzyme triple-staining protocols were tested with polyclonal and monoclonal antibodies on tissue sections and cytospin preparations. Various immunoconjugates were used in different combinations of methods, of which not all proved to be suitable. Of the tested protocols, one yielded superior results for both monoclonal and polyclonal antibodies, with optimal preservation of their original avidity. The method consists of a combination of indirect, direct, and avidin-biotin complex technique. The three antigens can be distinguished clearly and selectively by the reaction products of the enzyme activities of beta-galactosidase (green), alkaline phosphatase (blue), and horseradish peroxidase (red).

Volume 35, Issue 11, pp. 1199-1204, 11/01/1987
Copyright © 1987 by The Histochemical Society


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
O. Thaunat, A.-C. Field, J. Dai, L. Louedec, N. Patey, M.-F. Bloch, C. Mandet, M.-F. Belair, P. Bruneval, O. Meilhac, et al.
Lymphoid neogenesis in chronic rejection: Evidence for a local humoral alloimmune response
PNAS, October 11, 2005; 102(41): 14723 - 14728.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
C. M. van der Loos and H. Göbel
The Animal Research Kit (ARK) Can Be Used in a Multistep Double Staining Method for Human Tissue Specimens
J. Histochem. Cytochem., October 1, 2000; 48(10): 1431 - 1438.
[Abstract] [Full Text]

Home page
 J. Histochem. Cytochem.Home page
H.-A. Lehr, C. M. van der Loos, P. Teeling, and A. M. Gown
Complete Chromogen Separation and Analysis in Double Immunohistochemical Stains Using Photoshop-based Image Analysis
J. Histochem. Cytochem., January 1, 1999; 47(1): 119 - 126.
[Abstract] [Full Text]

Home page
 CirculationHome page
M. M.H. Marijianowski, M. van Laar, J. Bras, and A. E. Becker
Chronic Congestive Heart Failure Is Associated With a Phenotypic Shift of Intramyocardial Endothelial Cells
Circulation, September 15, 1995; 92(6): 1494 - 1498.
[Abstract] [Full Text]



Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1987