|
|
|
|
 |
|||
|
|||
Preservation of thrombomodulin antigen on vascular and extravascular surfaces
MC Boffa, B Burke and CC Haudenschild
Department of Pathology, Boston University School of Medicine, Mallory Institute of Pathology, Massachusetts 02118.
The protein C anticoagulant system is mediated by thrombin and is highly accelerated by thrombomodulin. We studied the distribution of thrombomodulin antigen (TM Ag) in the rabbit using an affinity-purified antibody raised in a goat against rabbit thrombomodulin. The preservation of TM Ag was highly dependent on immediate fixation of the surface on which it is located. TM Ag was found on the endothelium of the entire vasculature, whereas it was absent from all connective tissue, smooth and striated muscle, secretory epithelia, cartilage, bone, neural tissue, and all parenchyma examined. A new finding was the presence of TM Ag on nonvascular surfaces of body cavities (the mesothelia of pleura, pericardium, and peritoneum, the synovial membrane, and the arachnoid enveloping the central nervous system). By use of a functional assay, TM activity was recovered in buffered saline/detergent solution which was either injected into the intraperitoneal cavity of rabbits in vivo or incubated with the surface of the arachnoid in vitro. These findings extend the importance of anticoagulant mechanisms to the systems of slowly circulating fluids, in which they might be required for maintenance of the flow, and to mesothelial cavities, in which they could be necessary for preventing adherence between the surfaces, in conditions associated with pathological exudation.
Volume 35,
Issue 11,
pp. 1267-1276,
11/01/1987
Copyright © 1987 by The Histochemical Society
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  C.-S. Shi, G.-Y. Shi, Y.-S. Chang, H.-S. Han, C.-H. Kuo, C. Liu, H.-C. Huang, Y.-J. Chang, P.-S. Chen, and H.-L. Wu Evidence of Human Thrombomodulin Domain as a Novel Angiogenic Factor Circulation, April 5, 2005; 111(13): 1627 - 1636. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H.-C. Huang, G.-Y. Shi, S.-J. Jiang, C.-S. Shi, C.-M. Wu, H.-Y. Yang, and H.-L. Wu Thrombomodulin-mediated Cell Adhesion: INVOLVEMENT OF ITS LECTIN-LIKE DOMAIN J. Biol. Chem., November 21, 2003; 278(47): 46750 - 46759. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Ikeda, H. Ishii, T. Higuchi, K. Sato, Y. Hayashi, K. Ikeda, and Y. Hirabayashi Localization of Thrombomodulin in the Anterior Segment of the Human Eye Invest. Ophthalmol. Vis. Sci., October 1, 2000; 41(11): 3383 - 3390. [Abstract] [Full Text]
|
|
|
|
 |
|
 E. M. Conway, S. Pollefeyt, J. Cornelissen, I. DeBaere, M. Steiner-Mosonyi, J. I. Weitz, H. Weiler-Guettler, P. Carmeliet, and D. Collen Structure-Function Analyses of Thrombomodulin by Gene-Targeting in Mice: The Cytoplasmic Domain Is Not Required for Normal Fetal Development Blood, May 15, 1999; 93(10): 3442 - 3450. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Tohda, K. Oida, Y. Okada, S. Kosaka, E. Okada, S. Takahashi, H. Ishii, and I. Miyamori Expression of Thrombomodulin in Atherosclerotic Lesions and Mitogenic Activity of Recombinant Thrombomodulin in Vascular Smooth Muscle Cells Arterioscler. Thromb. Vasc. Biol., December 1, 1998; 18(12): 1861 - 1869. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Healy, W. W. Hancock, P. D. Christie, H. B. Rayburn, and R. D. Rosenberg Intravascular Coagulation Activation in a Murine Model of Thrombomodulin Deficiency: Effects of Lesion Size, Age, and Hypoxia on Fibrin Deposition Blood, December 1, 1998; 92(11): 4188 - 4197. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M-C. Boffa and M. Karmochkine Review : Thrombomodulin: an overview and potential implications in vascular disorders Lupus, January 1, 1998; 7(2_suppl): S120 - S125. [Abstract] [PDF]
|
|
|
|
 |
|
 H Weiler-Guettler, W. Aird, H Rayburn, M Husain, and R. Rosenberg Developmentally regulated gene expression of thrombomodulin in postimplantation mouse embryos Development, July 1, 1996; 122(7): 2271 - 2281. [Abstract] [PDF]
|
|
|
|
 |
|
 H. Weiler-Guettler, W. C. Aird, M. Husain, H. Rayburn, and R. D. Rosenberg Targeting of Transgene Expression to the Vascular Endothelium of Mice by Homologous Recombination at the Thrombomodulin Locus Circ. Res., February 1, 1996; 78(2): 180 - 187. [Abstract] [Full Text]
|
|
|
|
|
|
M-C. Boffa Thrombomodulin in human brain microvasculature Lupus, April 1, 1995; 4(2): 165 - 166. [PDF]
|
|
|
|
 |
|
 T. Malm and S. Bowald Antiadhesive membranes for cardiac reoperations J. Thorac. Cardiovasc. Surg., February 1, 1994; 107(2): 628 - 629. [Full Text]
|
|
| Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact |