|
|
|
|
 |
|||
|
|||
Immunohistochemical studies on hemoglobin-haptoglobin and hemoglobin catabolism sites
K Kino, K Mizumoto, J Watanabe and H Tsunoo
In order to clarify the catabolism sites of Hb-Hp and free Hb, the organ distributions of [125I]-Hb-Hp and [125I]-Hb were studied, and the cell types in each organ incorporating them were determined by immunohistochemical methods. After administration of [125I]-Hb-Hp in very small amounts to rats, 84.5% was incorporated into the liver, but the renal uptake was only 0.6%. [125I]-Hb was incorporated into the kidneys rather than into the liver when a fivefold greater amount of [125I]-Hb than the binding capacity of plasma Hp was administered. Parenchymal cells, but not Kupffer cells, in the liver were stained with anti-Hb or anti-Hp IgG after administration of Hb in an amount corresponding to the Hb binding capacity of Hp. The proximal tubule cells, but not the distal tubule cells, in the kidney were stained with anti-Hb IgG after administration of a fivefold greater amount of Hb than the binding capacity of Hp. On the basis of these results, we suggest that Hb-Hp was incorporated mainly into liver parenchymal cells and did not traverse glomeruli in the kidney. In contrast to Hb-Hp, free Hb could pass through the glomeruli easily and was incorporated into the proximal tubule cells.
Volume 35,
Issue 3,
pp. 381-386,
03/01/1987
Copyright © 1987 by The Histochemical Society
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  E. C. Y. Chow, L. Liu, N. Ship, R. H. Kluger, and K. S. Pang Role of Haptoglobin on the Uptake of Native and {beta}-Chain [Trimesoyl-(Lys82){beta}-(Lys82){beta}] Cross-Linked Human Hemoglobins in Isolated Perfused Rat Livers Drug Metab. Dispos., May 1, 2008; 36(5): 937 - 945. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. J. Ship, A. Toprak, R. P. Lai, E. Tseng, R. Kluger, and K. S. Pang Binding of acellular, native and cross-linked human hemoglobins to haptoglobin: enhanced distribution and clearance in the rat Am J Physiol Gastrointest Liver Physiol, June 1, 2005; 288(6): G1301 - G1309. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Gburek, P. J. Verroust, T. E. Willnow, J. C. Fyfe, W. Nowacki, C. Jacobsen, S. K. Moestrup, and E. I. Christensen Megalin and Cubilin are Endocytic Receptors Involved in Renal Clearance of Hemoglobin J. Am. Soc. Nephrol., February 1, 2002; 13(2): 423 - 430. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S.-K. Lim, H. Kim, S. K. Lim, A. b. Ali, Y. K. Lim, Y. Wang, S. M. Chong, F. Costantini, and H. Baumman Increased Susceptibility in Hp Knockout Mice During Acute Hemolysis Blood, September 15, 1998; 92(6): 1870 - 1877. [Abstract] [Full Text] [PDF]
|
|
| Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact |