Simultaneous light and electron microscopic observation of immunolabeled liver 27 KD gap junction protein on ultra-thin cryosectionsR Dermietzel, B Yancey, U Janssen-Timmen, O Traub, K Willecke and JP Revel
We report on immunolabeling of gap junction protein in rat liver. Simultaneous light and electron microscopic immunolabeling of ultra- thin frozen sections was performed to confirm that the antigenic targets of polyclonal antibodies and a monoclonal 27 KD antibody (12/1 C5) are the gap junctions. Our results clearly demonstrate that the immunoreactive sites determined by indirect immunofluorescence correspond to immunogold-labeled gap junctions identified in the same section according to electron microscopic criteria. Our results also support the concept that the 27 KD protein is a major constituent of gap junctions.
Volume 35,
Issue 3,
pp. 387-392,
03/01/1987
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M. L. Cotrina, J. H.-C. Lin, and M. Nedergaard Cytoskeletal Assembly and ATP Release Regulate Astrocytic Calcium Signaling J. Neurosci., November 1, 1998; 18(21): 8794 - 8804. [Abstract] [Full Text] [PDF] |
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Y Wang and B Rose An inhibition of gap-junctional communication by cadherins J. Cell Sci., January 2, 1997; 110(3): 301 - 309. [Abstract] [PDF] |
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Y. Wang, P. P. Mehta, and , B. Rose Inhibition of Glycosylation Induces Formation of Open Connexin-43 Cell-to-Cell Channels and Phosphorylation and Triton X-100 Insolubility of Connexin-43 J. Biol. Chem., November 3, 1995; 270(44): 26581 - 26585. [Abstract] [Full Text] [PDF] |
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Y Wang and B Rose Clustering of Cx43 cell-to-cell channels into gap junction plaques: regulation by cAMP and microfilaments J. Cell Sci., January 11, 1995; 108(11): 3501 - 3508. [Abstract] [PDF] |
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