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Immunohistochemical localization of acetylcholine receptors at human endplates using a monoclonal antibody

LM Smit, H Veldman and FG Jennekens

We describe a simple indirect immunohistochemical method for localization of acetylcholine receptors (AChR) in motor endplates at the light and electron microscopic level. This method involves the use of a monoclonal antibody directed against the main immunogenic region (MIR) of AChRs and is applicable to periodate-lysine-paraformaldehyde (PLP)-fixed tissue. We discuss the advantages of this method, as compared with the alpha-bungarotoxin-immunoperoxidase technique, and stress its value for diagnostic investigations of motor point biopsies from patients with neuromuscular transmission disorders.

Volume 35, Issue 5, pp. 613-617, 05/01/1987
Copyright © 1987 by The Histochemical Society


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