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Immunocytochemical detection of anti-mullerian hormone in Sertoli cells of various mammalian species including human
D Tran, JY Picard, J Campargue and N Josso
An immunocytochemical method, based on the use of a polyclonal antibody raised against purified bovine anti-Mullerian hormone (AMH), was used to detect AMH in Sertoli cell cytoplasm of various mammalian species, including human. Immunopurification of antiserum by AMH-affinity chromatography, although not mandatory, leads to better results and increased sensitivity. In human testicular tissue, AMH is detectable up to 6 years of age. In rats, AMH production is initiated at 13 days post coitum, peaks between 15 and 17 days, and is no longer detectable 1 week after birth. The reaction is strongest in Sertoli cells of calves, sheep, goats, and pigs, species characterized by a high degree of development of the rough endoplasmic reticulum. It is fainter in human, rat, rabbit, and cat Sertoli cells, in which the rough endoplasmic reticulum is not as abundant. This correlation is not unexpected, in view of the localization of reaction product in this cytoplasmic organelle. Preliminary results indicate that there may be a relationship between the amount of immunoreactive AMH present in testicular biopsies of intersex patients and the degree of regression of the Mullerian duct on the ipsilateral side. This may help to elucidate whether persistence of Mullerian ducts results from lack of testicular production of AMH or from peripheral resistance of the Mullerian primordia to the hormone.
Volume 35,
Issue 7,
pp. 733-743,
07/01/1987
Copyright © 1987 by The Histochemical Society
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