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Immunohistochemical antigen demonstration in plastic-embedded lymphoid tissue

H van Goor, G Harms, PO Gerrits, FG Kroese, S Poppema and J Grond

Department of Pathology, University of Groningen, The Netherlands.

We describe a method for post-embedding immunohistochemical demonstration of a wide range of antigens in glycol methacrylate- embedded tissue. Rat spleen and thymus tissues were fixed by immersion in fixatives containing different concentrations of paraformaldehyde, washed in sucrose phosphate buffer, dehydrated in acetone, infiltrated in a glycol methacrylate mixture in which the commonly used softener 2- butoxyethanol was replaced by butaandiol monoacrylate, and embedded. Trypsin was used to re-expose the masked antigenicity. Excellent results were obtained with a panel of monoclonal antibodies (MoAbs) directed against T-cells, B-cells, Ia-positive cells, macrophages, follicular dendritic cells, and leucocyte common antigen-bearing cells. The method described combines exact localization of antigens with optimal tissue morphology.

Volume 36, Issue 1, pp. 115-120, 01/01/1988
Copyright © 1988 by The Histochemical Society


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