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Differential inhibition of indirect immunofluorescence in rat liver sections incubated with antibodies against microsomal cytochromes P- 450a, b, and c and epoxide hydrolase

DE Moody, LA Taylor and EA Smuckler

Department of Pathology, School of Medicine, University of California, San Francisco 94143.

Rat liver sections were incubated with antibodies (100-1000 micrograms IgG/ml) against microsomal cytochromes P-450a, P-450b, and P-450c, and epoxide hydrolase. Inhibition of indirect immunofluorescence, which progressed with higher concentrations of primary antibody, corresponded with antigen-enriched tissue in frozen liver sections from male and female rats. It was found in liver sections from phenobarbital-treated rats incubated with anti-P-450b and anti-epoxide hydrolase and from 3- methylcholanthrene-treated rats incubated with anti-P-450c. No inhibition was found in sections from untreated rats or rats receiving treatments that did not induce the specific antigen. No inhibition was found in sections incubated with anti-P-450a. Inhibition of immunofluorescence was abolished in frozen sections subjected to dehydration-rehydration protocols known to extract antigens, and was prevented by certain solvents and detergent-wash. Inhibition of immunofluorescence provides a unique method for confirming the antigen- rich regions of the liver lobules specific for microsomal expoxide hydrolase and the cytochrome P-450s.

Volume 36, Issue 3, pp. 291-295, 03/01/1988
Copyright © 1988 by The Histochemical Society


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