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PAP labeling enhancement by osmium tetroxide-potassium ferrocyanide treatment

EF Lascano and MI Berria

Departamento de Microbiologia, Facultad de Medicina, Universidad de Buenos Aires, Argentina.

We describe a procedure to intensify staining of antigens labeled by the peroxidase-antiperoxidase (PAP) method. Routinely PAP-stained samples were enhanced by application of 5% osmium tetroxide for 30 min followed by freshly prepared 2% potassium ferrocyanide, plus 1% hydrochloric acid for 15 min. The staining color changed from the original golden-brown, through transient gray, to a very dark brown that was almost black. In addition to stronger labeling, antigen locations not apparent in untreated specimens may thus be disclosed. Furthermore, retrospective staining intensification can be performed in stored PAP-labeled samples even years later.

Volume 36, Issue 6, pp. 697-699, 06/01/1988
Copyright © 1988 by The Histochemical Society


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The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1988