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Light microscopic immunocytochemical demonstration of thyroid- stimulating hormone (TSH) receptors on normal rat thyroid cells

KW Schmid, B Jasani, JM Morgan and ED Williams

Department of Pathology, University of Wales College of Medicine, Heath Park, Cardiff, United Kingdom.

We present a method for immunohistochemical demonstration of surface- associated thyroid-stimulating hormone (TSH) receptors on direct imprints of rat thyroid follicular cells at the light microscopic level, by use of dinitrophenyl (DNP)-labeled bovine TSH as the primary probe in a DNP-hapten sandwich staining (DHSS) procedure. The light microscopically invisible diaminobenzidine (DAB) product of the enzyme reaction was amplified with a DAB enhancement technique (silver amplification), which yielded a reliable and distinct light microscopically visible cell surface staining. The consistently negative control results after inhibition with TSH receptor-specific reagents provide evidence in support of immunohistochemical localization of surface membrane-associated TSH receptors.

Volume 36, Issue 8, pp. 977-982, 08/01/1988
Copyright © 1988 by The Histochemical Society


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