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Immunocytochemical identification of bovine Langerhans cells by use of a monoclonal antibody directed against class II MHC antigens

LA Bryan, PJ Griebel, DM Haines, WC Davis and JR Allen

Department of Veterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Canada.

We undertook a study to develop a reliable light microscopic technique for identifying Langerhans cells (LC) in bovine epidermis. Monoclonal antibodies (MCA) detecting bovine class II MHC antigens were used in conjunction with an avidin-biotin-peroxidase complex (ABC) immunocytochemical staining method. The specificity of the MCA for LC was confirmed ultrastructurally by use of gold-labeled second antibody. Epidermal sheets and epidermal single-cell suspensions examined by light microscopy confirm that bovine epidermal LC express class II antigens. Anti-bovine class II MCA is a dependable reagent for identification of LC in normal bovine epidermis.

Volume 36, Issue 8, pp. 991-995, 08/01/1988
Copyright © 1988 by The Histochemical Society


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