|
|
|
|
 |
|||
|
|||
Immunohistochemical localization of Na+,K+-ATPase in rodent and human salivary and lacrimal glands
DC Winston, RA Hennigar, SS Spicer, JR Garrett and BA Schulte
Department of Pathology, Medical University of South Carolina, Charleston 29425.
The enzyme Na+,K+-ATPase was localized immunohistochemically in major salivary glands of mouse, rat, and human and in exorbital lacrimal glands of the rodents. Immunoreactive Na+,K+-ATPase was abundant in the basolateral membranes of all epithelial cells lining striated and intra- and interlobular ducts of all glands. Reactivity of intercalated ducts varied among gland type and species. Cells lining granular ducts in rodent submandibular gland showed a heterogeneous staining pattern in rat but stained homogeneously in mouse. Secretory cells varied greatly in their content of immunoreactive Na+,K+-ATPase. As with all duct cells, staining was present only at the basolateral surface and was never observed at the luminal surface of reactive secretory cells. Mucous cells failed to show any reactivity in any gland examined. Serous cells showed a gradient of immunostaining intensity ranging from strongly positive in demilunes of human sublingual gland to negative in rat submandibular gland and lacrimal glands of rats and mice. The presence of basolaterally localized Na+,K+-ATPase in most serous cells but not in mucous cells suggests that the enzyme contributes to the ion and water content of copious, low-protein serous secretions. The intense immunostaining of cells in most if not all segments of the duct system supports the idea that the ducts are involved with modification of the primary saliva, and extends this concept to include all segments of the duct system.
Volume 36,
Issue 9,
pp. 1139-1145,
09/01/1988
Copyright © 1988 by The Histochemical Society
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  T. Nakamoto, A. Srivastava, V. G. Romanenko, C. E. Ovitt, P. Perez-Cornejo, J. Arreola, T. Begenisich, and J. E. Melvin Functional and molecular characterization of the fluid secretion mechanism in human parotid acinar cells Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2007; 292(6): R2380 - R2390. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H.-J. Lee, Y.-J. Lee, H.-C. Kwon, S. Bae, S.-H. Kim, J.-J. Min, C.-K. Cho, and Y.-S. Lee Radioprotective Effect of Heat Shock Protein 25 on Submandibular Glands of Rats Am. J. Pathol., November 1, 2006; 169(5): 1601 - 1611. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. L. Ubels, H. M. Hoffman, S. Srikanth, J. H. Resau, and C. P. Webb Gene Expression in Rat Lacrimal Gland Duct Cells Collected Using Laser Capture Microdissection: Evidence for K+ Secretion by Duct Cells Invest. Ophthalmol. Vis. Sci., May 1, 2006; 47(5): 1876 - 1885. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Gresz, T.-H. Kwon, H. Gong, P. Agre, M. C. Steward, L. S. King, and S. Nielsen Immunolocalization of AQP-5 in rat parotid and submandibular salivary glands after stimulation or inhibition of secretion in vivo Am J Physiol Gastrointest Liver Physiol, July 1, 2004; 287(1): G151 - G161. [Abstract] [Full Text] [PDF]
|
|
| Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact |