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An immunohistochemical study performed with monoclonal and polyclonal antibodies to mouse epidermal growth factor

HJ Beerstecher, C Huiskens-van der Meij and SO Warnaar

Department of Pathology, University of Leiden, The Netherlands.

We developed two monoclonal antibodies, E5 and F5, which react with mouse epidermal growth factor (mEGF) and urogastrone. These antibodies and a rabbit antiserum to mEGF were used for immunohistochemical analysis of staining reactions in rodent and human tissues. Our results do not confirm the published reports of EGF-like immunoreactivity in Brunner's glands, human submandibular glands, human kidney tissue, or rodent brain sections.

Volume 36, Issue 9, pp. 1153-1160, 09/01/1988
Copyright © 1988 by The Histochemical Society


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