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Detection of diaminobenzidine reactions using scanning laser confocal reflectance microscopy

JM Robinson and BE Batten

Department of Anatomy, Ohio State University, Columbus 43210.

We used scanning laser confocal microscopy to visualize sites of peroxidatic activity as detected by the diaminobenzidine (DAB) reaction. Imaging was achieved by employing the reflectance mode of this instrument. Intense reflectance was detected after DAB localization of endogenous granule-associated myeloperoxidase in neutrophils and of the exogenous tracer horseradish peroxidase in mouse oocytes. Detection of DAB reaction products with confocal reflectance microscopy will probably be an important addition to the utility of this cytochemical technique.

Volume 37, Issue 12, pp. 1761-1765, 12/01/1989
Copyright © 1989 by The Histochemical Society


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