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Method for epitope selection of antisera for immunohistology

D Wedlich

Institut fur Molekularbiologie und Biochemie, Freie Universitat Berlin, FRG.

Polyclonal anti-laminin serum was affinity-purified on paraformaldehyde- fixed laminin on a nitrocellulose filter. The purified antibodies were tested for their specificity in immunohistological stainings on frozen sections of paraformaldehyde-fixed tissue. As compared to the initial polyclonal serum, the purified antibodies increased the specificity of antigen detection, since all background caused by nonspecific reactions was eliminated. This technique promises to be very useful for immunohistological analysis using light and electron microscopy.

Volume 37, Issue 2, pp. 273-276, 02/01/1989
Copyright © 1989 by The Histochemical Society


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The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1989