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Synthesis of a more stable osmium ammine electron-dense DNA stain

AL Olins, BA Moyer, SH Kim and DP Allison

University of Tennessee-Oak Ridge, Graduate School of Biomedical Sciences.

Specific DNA staining for electron microscopic observation is simplified by a shorter synthesis of the staining reagent. The new, more reliable reagent, osmium ammine-B, is stable for more than a year, dissolves completely in water, and does not require reoptimization of staining conditions for every batch, yet reproducibly gives strong contrast to DNA-containing structures.

Volume 37, Issue 3, pp. 395-398, 03/01/1989
Copyright © 1989 by The Histochemical Society


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