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Binding of biotin to hepatitis B surface antigen: a possible pitfall in immunohistochemistry

JJ van den Oord, F Facchetti, C de Wolf-Peeters and VJ Desmet

Department of Pathology, University Hospital Sint Rafael, Catholic University of Leuven, Belgium.

We report on the binding of biotin, and hence of biotinylated antibodies and lectins, to ground glass hepatocytes and liver cell membranes in chronic hepatitis B viral infection. This binding is of low affinity, and was proved to be directed at the hepatitis B surface antigen, presumably at its disulfide bonds. To avoid false-positive results, this affinity should be considered in the interpretation of immunohistochemical stainings of hepatitis B virus-infected liver tissue with biotinylated reagents.

Volume 37, Issue 4, pp. 551-554, 04/01/1989
Copyright © 1989 by The Histochemical Society


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The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1989