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Marked and variable inhibition by chemical fixation of cytochrome oxidase and succinate dehydrogenase in single motoneurons

GR Chalmers and VR Edgerton

Department of Kinesiology, University of California, Los Angeles 90024.

The effect of tissue fixation on succinate dehydrogenase and cytochrome oxidase activity in single motoneurons of the rat was demonstrated using a computer image processing system. Inhibition of enzyme activity by chemical fixation was variable, with some motoneurons being affected more than others. It was concluded that quantification of enzymatic activity in chemically fixed tissue provides an imprecise estimate of enzyme activities found in fresh-frozen tissues.

Volume 37, Issue 6, pp. 899-901, 06/01/1989
Copyright © 1989 by The Histochemical Society


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