Marked and variable inhibition by chemical fixation of cytochrome oxidase and succinate dehydrogenase in single motoneuronsGR Chalmers and VR Edgerton Department of Kinesiology, University of California, Los Angeles 90024. The effect of tissue fixation on succinate dehydrogenase and cytochrome oxidase activity in single motoneurons of the rat was demonstrated using a computer image processing system. Inhibition of enzyme activity by chemical fixation was variable, with some motoneurons being affected more than others. It was concluded that quantification of enzymatic activity in chemically fixed tissue provides an imprecise estimate of enzyme activities found in fresh-frozen tissues.
Volume 37,
Issue 6,
pp. 899-901,
06/01/1989
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T. Adachi, N. Kikuchi, K. Yasuda, R. Anahara, N. Gu, T. Matsunaga, T. Yamamura, C. Mori, G. Tsujimoto, K. Tsuda, et al. Muscle: Fibre type distribution and gene expression levels of both succinate dehydrogenase and peroxisome proliferator-activated receptor-{gamma} coactivator-1{alpha} of fibres in the soleus muscle of Zucker diabetic fatty rats Exp Physiol, March 1, 2007; 92(2): 449 - 455. [Abstract] [Full Text] [PDF] |
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J. Valla, J. D. Berndt, and F. Gonzalez-Lima Energy Hypometabolism in Posterior Cingulate Cortex of Alzheimer's Patients: Superficial Laminar Cytochrome Oxidase Associated with Disease Duration J. Neurosci., July 1, 2001; 21(13): 4923 - 4930. [Abstract] [Full Text] [PDF] |
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