|
|
|
|
 |
|||
|
|||
Immunocytochemical identification of cell types in human mammary gland: variations in cellular markers are dependent on glandular topography and differentiation
PS Rudland and CM Hughes
Department of Biochemistry, University of Liverpool, United Kingdom.
Antiserum to epithelial membrane antigen and three monoclonal antibodies (MAb) to milk-fat globule membranes immunocytochemically stain only epithelial cells, whereas a fourth reacts also with myoepithelial cells in inter- and intralobular ducts of human breast. Staining with peanut lectin shows a gradual increase for epithelial cells, from little or no staining in ducts through variable staining in ductules to intense staining in secretory alveoli. Antisera and MAb to vimentin, smooth-muscle actin, MAb to the common acute lymphoblastic leukemia antigen and to a glycoprotein of 135 KD stain myoepithelial cells in main ducts, but this staining is reduced in inter- and intralobular ducts and ductules. MAb to epithelial-specific keratin 18 stain a minor population of ductal epithelial cells, the major population of epithelial cells in interlobular (ILD) and extralobular terminal ducts (ETD), and epithelial cells in a minority of ductules. In lactating glands most epithelial cells in ductules are stained, but the alveolar and myoepithelial cells are unstained. Keratin MAb PKK2 and LP34 strongly stain myoepithelial cells, but only a minor population of epithelial cells in main ducts. However, these MAb stain principally the epithelial cells in ILD, ETD, and a minority of ductules. In lactating glands most epithelial cells are stained in ductules, but the myoepithelial and not the alveolar cells are stained intensely in secretory lobules. It is suggested that the unusual staining pattern of cells found principally in the ILD, ETD, and some ductules may represent regions of growth and/or subpopulation(s) of cells intermediate between epithelial and myoepithelial cells.
Volume 37,
Issue 7,
pp. 1087-1100,
07/01/1989
Copyright © 1989 by The Histochemical Society
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  Y. Sun, K. Boyd, W. Xu, J. Ma, C. W. Jackson, A. Fu, J. M. Shillingford, G. W. Robinson, L. Hennighausen, J. K. Hitzler, et al. Acute Myeloid Leukemia-Associated Mkl1 (Mrtf-a) Is a Key Regulator of Mammary Gland Function Mol. Cell. Biol., August 1, 2006; 26(15): 5809 - 5826. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Gama, A. Alves, F. Gartner, and F. Schmitt p63: A Novel Myoepithelial Cell Marker in Canine Mammary Tissues Vet. Pathol., July 1, 2003; 40(4): 412 - 420. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. E. Arango, P. Li, M. Komatsu, C. Montes, C. A. C. Carraway, and K. L. Carraway Production and Localization of Muc4/Sialomucin Complex and Its Receptor Tyrosine Kinase ErbB2 in the Rat Lacrimal Gland Invest. Ophthalmol. Vis. Sci., November 1, 2001; 42(12): 2749 - 2756. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. S. Rudland, A. Platt-Higgins, C. Renshaw, C. R. West, J. H. R. Winstanley, L. Robertson, and R. Barraclough Prognostic Significance of the Metastasis-inducing Protein S100A4 (p9Ka) in Human Breast Cancer Cancer Res., March 1, 2000; 60(6): 1595 - 1603. [Abstract] [Full Text]
|
|
|
|
 |
|
 M. L. Ackland, P. Anikijenko, A. Michalczyk, and J. F.B. Mercer Expression of Menkes Copper-transporting ATPase, MNK, in the Lactating Human Breast: Possible Role in Copper Transport into Milk J. Histochem. Cytochem., December 1, 1999; 47(12): 1553 - 1562. [Abstract] [Full Text]
|
|
|
|
 |
|
 G. Robinson, R. McKnight, G. Smith, and L Hennighausen Mammary epithelial cells undergo secretory differentiation in cycling virgins but require pregnancy for the establishment of terminal differentiation Development, January 7, 1995; 121(7): 2079 - 2090. [Abstract] [PDF]
|
|
| Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact |