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Ethidium bromide- and propidium iodide-PTA staining of nucleic acids at the electron microscopic level
M Biggiogera and FF Biggiogera
Centre de Microscopie Electronique de l'Universite de Lausanne, Switzerland.
Ultra-thin sections of various tissues were stained with ethidium bromide or propidium iodide, two fluorescent markers widely used for quantitation of nucleic acids. The fluorochromes, tested at different concentrations, were then revealed by incubation of the sections with neutralized phosphotungstic acid. We showed that at the electron microscopic level only nucleic acid-containing structures are revealed. Chromatin, nucleolus, and ribosomes appear to be stained by the end- product of the reaction. Furthermore, controls with proteases and nucleases showed that the staining is related to the binding of the fluorochromes to DNA and RNA and to the subsequent detection of the dyes by neutralized PTA.
Volume 37,
Issue 7,
pp. 1161-1166,
07/01/1989
Copyright © 1989 by The Histochemical Society
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