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Cortical granule-specific components are present within oocytes and accessory cells during sea urchin oogenesis

GM Wessel

Department of Biochemistry and Molecular Biology, University of Texas MD Anderson Cancer Center, Houston 77030.

The coordinate expression of cortical granule-specific components in sea urchin oogenesis was studied using antibody probes. The components used to generate the organelle-specific antibodies included the whole cortical granule exudate, fertilization envelopes, hyalin, beta, 1- 3,glucanase, and Ig8. Using immunolocalization techniques at both the light and electron microscopic levels, these molecules were found to be specific to cortical granules in three distinct cell types: developing oocytes, eggs, and accessory cells. In early oocytes, each of the cortical granule components are coordinately accumulated in the developing cortical granules dispersed throughout the cytoplasm. No other organelle within the developing oocytes or eggs contained detectable levels of any of these epitopes. In the somatic interstitial tissue of the ovary, cortical granule components also were accumulated specifically within cortical granule structures. Found only in select accessory cells, these cortical granules were indistinguishable in morphology and epitope composition from eggs and were contained within cytoplasmic aggregates termed mosaic globules. The mechanism of cortical granule concentration into mosaic globules is unknown, but this demonstration of common organelle constituents between oocytes and accessory cells may provide insight for such an understanding.

Volume 37, Issue 9, pp. 1409-1420, 09/01/1989
Copyright © 1989 by The Histochemical Society


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This article has been cited by other articles:


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V.S. Gross, G. Wessel, H.M. Florman, and T. Ducibella
A Monoclonal Antibody That Recognizes Mammalian Cortical Granules and a32-Kilodalton Protein in Mouse Eggs
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[Abstract] [Full Text]

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 DevelopmentHome page
M Laidlaw and G. Wessel
Cortical granule biogenesis is active throughout oogenesis in sea urchins
Development, January 5, 1994; 120(5): 1325 - 1333.
[Abstract] [PDF]



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