Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mazzotti, G.
Right arrow Articles by Maraldi, N. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mazzotti, G.
Right arrow Articles by Maraldi, N. M.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

High-resolution detection of newly synthesized DNA by anti- bromodeoxyuridine antibodies identifies specific chromatin domains

G Mazzotti, R Rizzoli, A Galanzi, S Papa, M Vitale, M Falconi, LM Neri, N Zini and NM Maraldi

Istituto di Anatomia Umana Normale, Universita di Bologna, Italy.

We analyzed the incorporation of bromodeoxyuridine (BrdUrd) into DNA in exponentially growing murine erythroleukemia cells (FLC-745), using fluorescent anti-BrdUrd antibodies with light microscopy and flow cytometry. The fine localization of the DNA replicating sites was investigated at the ultrastructural level by using a second antibody conjugated with colloidal gold. The latter approach, which does not require acidic denaturation of the DNA, enables preservation of good morphology and obtains a better resolution power than that of electron microscopic autoradiography, the percentage of labeled cells obtained with the two techniques being comparable. After short BrdUrd pulses, characteristic distribution of the labeling can be identified in the heterochromatin, in interchromatin domains, or at the boundary between the dispersed and the condensed chromatin. Similar patterns are also observable in the nuclear structures which condense after acid denaturation, suggesting that DNA replication takes place at fixed sites associated with the nuclear matrix.

Volume 38, Issue 1, pp. 13-22, 01/01/1990
Copyright © 1990 by The Histochemical Society


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Histochem. Cytochem.Home page
G. Lattanzi, A. Galanzi, P. Gobbi, M. Falconi, A. Matteucci, L. Breschi, M. Vitale, and G. Mazzotti
Ultrastructural Aspects of the DNA Polymerase {alpha} Distribution During the Cell Cycle
J. Histochem. Cytochem., December 1, 1998; 46(12): 1435 - 1442.
[Abstract] [Full Text]

Home page
 J. Cell Sci.Home page
J Ferreira and M Carmo-Fonseca
Genome replication in early mouse embryos follows a defined temporal and spatial order
J. Cell Sci., January 4, 1997; 110(7): 889 - 897.
[Abstract] [PDF]

Home page
 J. Cell Sci.Home page
P Hozak, D. Jackson, and P. Cook
Replication factories and nuclear bodies: the ultrastructural characterization of replication sites during the cell cycle
J. Cell Sci., January 8, 1994; 107(8): 2191 - 2202.
[Abstract] [PDF]

Home page
 J. Cell Sci.Home page
N. Maraldi, S Santi, N Zini, A Ognibene, R Rizzoli, G Mazzotti, R Di Primio, R Bareggi, V Bertagnolo, C Pagliarini, et al.
Decrease in nuclear phospholipids associated with DNA replication
J. Cell Sci., January 3, 1993; 104(3): 853 - 859.
[Abstract] [PDF]



Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1990