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Optimizing parameters for correlative immunogold localization by video- enhanced light microscopy, high-voltage transmission electron microscopy, and field emission scanning electron microscopy

SR Simmons, JB Pawley and RM Albrecht

Department of Veterinary Science, University of Wisconsin, Madison 53706.

Correlative video-enhanced light microscopy, high-voltage transmission electron microscopy, and low-voltage high resolution scanning electron microscopy were used to examine the binding of colloidal gold-labeled fibrinogen to platelet surfaces. Optimal conditions for the detection of large (18 nm) and small (3 nm) gold particles are described.

Volume 38, Issue 12, pp. 1781-1785, 12/01/1990
Copyright © 1990 by The Histochemical Society


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The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1990