Identification of a novel type 2 fiber population in mammalian skeletal muscle by combined use of histochemical myosin ATPase and anti-myosin monoclonal antibodies

L Gorza

Institute of General Pathology, University of Padova, Italy.

A novel type of myosin heavy chain (MHC), called 2X, has been recently identified in type 2 fibers of rat skeletal muscles using an immunochemical approach. In the present study, the same panel of anti- MHC monoclonal antibodies was used in immunohistochemistry combined with enzyme histochemistry to identify and compare type 2X fibers in hindlimb skeletal muscles of rat, mouse, and guinea pig. Immunohistochemistry shows that 2X MHC is localized in a large subset of type 2 fibers and is co-expressed with 2A or 2B MHC in a small number of fibers. Enzyme histochemistry shows that type 2X fibers display low myosin ATPase activity after pre-incubation at pH 4.3 and high activity after paraformaldehyde pre-incubation at pH 10.4. After pre-incubation at pH 4.6, myosin ATPase shows intermediate and high activity in rat and mouse 2X fibers, respectively, whereas it is low in guinea pig 2X fibers. Succinate dehydrogenase displays moderate to high activity in 2X fibers of all species. Taken together, these staining patterns allow this novel fiber population to be distinguished from the other type 2 fibers using only enzyme histochemistry. Nevertheless, the combined use of immuno- and enzyme histochemistry prevents incorrect fiber typing due to the interspecies variability of myosin ATPase activity among the correspondent fiber types, and completely modifies the presently used classification of mouse type 2 fibers.

Volume 38, Issue 2, pp. 257-265, 02/01/1990
Copyright © 1990 by The Histochemical Society

CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

This article has been cited by other articles:

				H. S. Rhee and J. F.Y. Hoh Immunohistochemical Analysis of Myosin Heavy Chain Expression in Laryngeal Muscles of the Rabbit, Cat, and Baboon J. Histochem. Cytochem., October 1, 2008; 56(10): 929 - 950. [Abstract] [Full Text] [PDF]

				S. Schiaffino, M. Sandri, and M. Murgia Activity-Dependent Signaling Pathways Controlling Muscle Diversity and Plasticity Physiology, August 1, 2007; 22(4): 269 - 278. [Abstract] [Full Text] [PDF]

				B. C. Harrison, D. L. Allen, B. Girten, L. S. Stodieck, P. J. Kostenuik, T. A. Bateman, S. Morony, D. Lacey, and L. A. Leinwand Skeletal muscle adaptations to microgravity exposure in the mouse J Appl Physiol, December 1, 2003; 95(6): 2462 - 2470. [Abstract] [Full Text]

				R. R. Watson, T. A. Miller, and R. W. Davis Immunohistochemical fiber typing of harbor seal skeletal muscle J. Exp. Biol., November 15, 2003; 206(22): 4105 - 4111. [Abstract] [Full Text] [PDF]

				Y. Totsuka, Y. Nagao, T. Horii, H. Yonekawa, H. Imai, H. Hatta, Y. Izaike, T. Tokunaga, and Y. Atomi Physical performance and soleus muscle fiber composition in wild-derived and laboratory inbred mouse strains J Appl Physiol, August 1, 2003; 95(2): 720 - 727. [Abstract] [Full Text] [PDF]

				M. I. Lewis, H. Li, Z.-S. Huang, M. S. Biring, B. Cercek, and M. Fournier Influence of varying degrees of malnutrition on IGF-I expression in the rat diaphragm J Appl Physiol, August 1, 2003; 95(2): 555 - 562. [Abstract] [Full Text] [PDF]

				M. Suwa, H. Nakano, Y. Higaki, T. Nakamura, S. Katsuta, and S. Kumagai Increased wheel-running activity in the genetically skeletal muscle fast-twitch fiber-dominant rats J Appl Physiol, January 1, 2003; 94(1): 185 - 192. [Abstract] [Full Text] [PDF]

				O. Mathieu-Costello, S. Morales, J. Savolainen, and M. Vornanen Fiber capillarization relative to mitochondrial volume in diaphragm of shrew J Appl Physiol, July 1, 2002; 93(1): 346 - 353. [Abstract] [Full Text] [PDF]

				L. Lefaucheur, P. Ecolan, L. Plantard, and N. Gueguen New Insights into Muscle Fiber Types in the Pig J. Histochem. Cytochem., May 1, 2002; 50(5): 719 - 730. [Abstract] [Full Text] [PDF]

				B. C. Harrison, M. L. Bell, D. L. Allen, W. C. Byrnes, and L. A. Leinwand Skeletal muscle adaptations in response to voluntary wheel running in myosin heavy chain null mice J Appl Physiol, January 1, 2002; 92(1): 313 - 322. [Abstract] [Full Text] [PDF]

				G. H. Graziotti, C. M. Rios, and J.-L. L. Rivero Evidence for Three Fast Myosin Heavy Chain Isoforms in Type II Skeletal Muscle Fibers in the Adult Llama (Lama glama) J. Histochem. Cytochem., July 1, 2001; 49(8): 1033 - 1044. [Abstract] [Full Text] [PDF]

				D. L. Allen, B. C. Harrison, A. Maass, M. L. Bell, W. C. Byrnes, and L. A. Leinwand Cardiac and skeletal muscle adaptations to voluntary wheel running in the mouse J Appl Physiol, May 1, 2001; 90(5): 1900 - 1908. [Abstract] [Full Text] [PDF]

				G. C. Sieck and M. Regnier Plasticity in Skeletal, Cardiac, and Smooth Muscle: Invited Review: Plasticity and energetic demands of contraction in skeletal and cardiac muscle J Appl Physiol, March 1, 2001; 90(3): 1158 - 1164. [Abstract] [Full Text] [PDF]

				D. L. Allen, B. C. Harrison, C. Sartorius, W. C. Byrnes, and L. A. Leinwand Mutation of the IIB myosin heavy chain gene results in muscle fiber loss and compensatory hypertrophy Am J Physiol Cell Physiol, March 1, 2001; 280(3): C637 - C645. [Abstract] [Full Text] [PDF]

				G. C. Sieck and W.-Z. Zhan Denervation alters myosin heavy chain expression and contractility of developing rat diaphragm muscle J Appl Physiol, September 1, 2000; 89(3): 1106 - 1113. [Abstract] [Full Text] [PDF]

				Y. S. Prakash, C. B. Mantilla, W.-Z. Zhan, K. G. Smithson, and G. C. Sieck Phrenic motoneuron morphology during rapid diaphragm muscle growth J Appl Physiol, August 1, 2000; 89(2): 563 - 572. [Abstract] [Full Text] [PDF]

				M. W. Berchtold, H. Brinkmeier, and M. Muntener Calcium Ion in Skeletal Muscle: Its Crucial Role for Muscle Function, Plasticity, and Disease Physiol Rev, July 1, 2000; 80(3): 1215 - 1265. [Abstract] [Full Text] [PDF]

				Y. Atomi, K. Toro, T. Masuda, and H. Hatta Fiber-type-specific alpha B-crystallin distribution and its shifts with T3 and PTU treatments in rat hindlimb muscles J Appl Physiol, April 1, 2000; 88(4): 1355 - 1364. [Abstract] [Full Text] [PDF]

				M. Fournier and M. I. Lewis Influences of IGF-I gene disruption on the cellular profile of the diaphragm Am J Physiol Endocrinol Metab, April 1, 2000; 278(4): E707 - E715. [Abstract] [Full Text] [PDF]

				J. J. Sciote and T. J. Morris Skeletal Muscle Function and Fibre Types: the Relationship Between Occlusal Function and the Phenotype of Jaw-closing Muscles in Human J. Orthod., March 1, 2000; 27(1): 15 - 30. [Abstract] [Full Text] [PDF]

				P. C. Geiger, M. J. Cody, and G. C. Sieck Force-calcium relationship depends on myosin heavy chain and troponin isoforms in rat diaphragm muscle fibers J Appl Physiol, November 1, 1999; 87(5): 1894 - 1900. [Abstract] [Full Text] [PDF]

				Skeletal Muscle Dysfunction in Chronic Obstructive Pulmonary Disease . A Statement of the American Thoracic Society and European Respiratory Society Am. J. Respir. Crit. Care Med., April 1, 1999; 159(4): S2 - 40. [Full Text] [PDF]

				M. I. Lewis, M. Fournier, A. Y. Yeh, P. E. Micevych, and G. C. Sieck Alterations in diaphragm contractility after nandrolone administration: an analysis of potential mechanisms J Appl Physiol, March 1, 1999; 86(3): 985 - 992. [Abstract] [Full Text] [PDF]

				M. I. Lewis, A. T. Feinberg, and M. Fournier IGF-I and/or growth hormone preserve diaphragm fiber size with moderate malnutrition J Appl Physiol, July 1, 1998; 85(1): 189 - 197. [Abstract] [Full Text] [PDF]

				M. S. Biring, M. Fournier, D. J. Ross, and M. I. Lewis Cellular adaptations of skeletal muscles to cyclosporine J Appl Physiol, June 1, 1998; 84(6): 1967 - 1975. [Abstract] [Full Text] [PDF]

				R. J. L. Murphy, P. F. Gardiner, G. Rousseau, M. Bouvier, and L. Beliveau Chronic beta -blockade increases skeletal muscle beta -adrenergicreceptor density and enhances contractile force J Appl Physiol, August 1, 1997; 83(2): 459 - 465. [Abstract] [Full Text] [PDF]

				M. I. Lewis, T. J. Lorusso, and M. Fournier Anabolic influences of insulin-like growth factor I and/or growth hormone on the diaphragm of young rats J Appl Physiol, June 1, 1997; 82(6): 1972 - 1978. [Abstract] [Full Text] [PDF]

				M. I. Lewis, T. J. Lorusso, and M. Fournier Effect of insulin-like growth factor I and/or growth hormone on diaphragm of malnourished adolescent rats J Appl Physiol, April 1, 1997; 82(4): 1064 - 1070. [Abstract] [Full Text] [PDF]

				W.-Z. Zhan, H. Miyata, Y. S. Prakash, and G. C. Sieck Metabolic and phenotypic adaptations of diaphragm muscle fibers with inactivation J Appl Physiol, April 1, 1997; 82(4): 1145 - 1153. [Abstract] [Full Text] [PDF]

				L. E. NECKING, R. LUNDSTROM, L. B. DAHLIN, G. LUNDBORG, L-E. THORNELL, and J. FRIDEN Tissue Displacement Is a Causative Factor in Vibration-Induced Muscle Injury J Hand Surg Eur Vol., December 1, 1996; 21(6): 753 - 757. [Abstract] [PDF]

				M Cho, S. Hughes, I Karsch-Mizrachi, M Travis, L. Leinwand, and H. Blau Fast myosin heavy chains expressed in secondary mammalian muscle fibers at the time of their inception J. Cell Sci., January 9, 1994; 107(9): 2361 - 2371. [Abstract] [PDF]

				M. J. Donoghue, B. L. Patton, J. R. Sanes, and J. P. Merlie An axial gradient of transgene methylation in murine skeletal muscle: genomic imprint of rostrocaudal position Development, December 1, 1992; 116(4): 1101 - 1112. [Abstract] [PDF]

				J.E. Bright, R.H. Inns, N.J. Tuckwell, G.D. Griffiths, and T.C. Marrs A Histochemical Study of Changes Observed in the Mouse Diaphragm after Organophosphate Poisoning Human and Experimental Toxicology, January 1, 1991; 10(1): 9 - 14. [Abstract] [PDF]

				P Sutrave, A M Kelly, and S H Hughes ski can cause selective growth of skeletal muscle in transgenic mice. Genes & Dev., September 1, 1990; 4(9): 1462 - 1472. [Abstract] [PDF]

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact

The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1990