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Localization of cerium-based reaction products by scanning laser reflectance confocal microscopy

JM Robinson and BE Batten

Department of Anatomy, Ohio State University, Columbus 43210.

Scanning laser confocal microscopy was utilized to visualize sites of hydrogen peroxide release from stimulated neutrophils and lysosomal acid phosphatase in these and other cells using cerium in the detection systems. Imaging of the cerium-containing reactions was achieved by employing the reflectance mode of this instrument. Localization of these products at the light microscope level was direct and did not require other reactions to generate a visible product. This new approach to cerium cytochemistry should prove useful for many applications.

Volume 38, Issue 3, pp. 315-318, 03/01/1990
Copyright © 1990 by The Histochemical Society


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