Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Laman, J. D.
Right arrow Articles by Claassen, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Laman, J. D.
Right arrow Articles by Claassen, E.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Double immunocytochemical staining for in vivo detection of epitope specificity and isotype of antibody-forming cells against synthetic peptides homologous to human immunodeficiency virus-1

JD Laman, K Gerritse, M Fasbender, WJ Boersma, N van Rooijen and E Claassen

Department of Immunology, TNO Medical Biological Laboratory, Rijswijk, The Netherlands.

Many infections evoke a strong humoral immune response. Some (e.g., HIV- 1, EBV, CMV) also lead to disorders of the B-cell system. Data concerning cell dysfunction are largely derived from in vitro studies, which necessarily exclude all microenvironmental influences. The aim of this study was to develop a tool for the investigation of epitope specific humoral immune responses in vivo. Mice were immunized with one of two synthetic peptides, both 21 amino acids long and homologous to regions of the HIV-1 gp160. Cryostat sections of spleen and lymph nodes were incubated with the corresponding peptide coupled to alkaline phosphatase and simultaneously incubated with peroxidase-conjugated rabbit antisera specific for mouse immunoglobulin isotypes. We were able to show simultaneous detection of epitope specificity, isotype, and localization of antibody-forming cells and immune complexes in tissue sections. It should prove useful for in vivo investigation of the development of specific (e.g., anti-HIV-1) humoral immune response, the determination of B-cell specificity in lymph node infiltrates, and the role of immune complexes in lymph node pathology.

Volume 38, Issue 4, pp. 457-462, 04/01/1990
Copyright © 1990 by The Histochemical Society


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Virol.Home page
D. H. Margolin, E. F. Helmuth Saunders, B. Bronfin, N. de Rosa, M. K. Axthelm, X. Alvarez, and N. L. Letvin
High Frequency of Virus-Specific B Lymphocytes in Germinal Centers of Simian-Human Immunodeficiency Virus-Infected Rhesus Monkeys
J. Virol., March 19, 2002; 76(8): 3965 - 3973.
[Abstract] [Full Text] [PDF]

Home page
 BrainHome page
I. A. Schrijver, M. van Meurs, M.-J. Melief, C. Wim Ang, D. Buljevac, R. Ravid, M. P. Hazenberg, and J. D. Laman
Bacterial peptidoglycan and immune reactivity in the central nervous system in multiple sclerosis
Brain, August 1, 2001; 124(8): 1544 - 1554.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
I. A. Schrijver, M.-J. Melief, M. van Meurs, A. R. Companjen, and J. D. Laman
Pararosaniline Fixation for Detection of Co-stimulatory Molecules, Cytokines, and Specific Antibody
J. Histochem. Cytochem., January 1, 2000; 48(1): 95 - 104.
[Abstract] [Full Text]



Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1990