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Association of adenosine deaminase with erythrocyte and platelet plasma membrane: an immunological study using light and electron microscopy

R Franco, JM Aran, D Colomer, E Matutes and JL Vives-Corrons

Department de Bioquimica i Fisiologia, Unitat de Bioquimica i Biologia Molecular A, Facultat de Quimica, Barcelona, Catalunya, Spain.

Adenosine deaminase has been localized in the plasma membrane of erythrocytes and platelets by means of immunological techniques using light and electron microscopy with cells in suspension. In erythrocytes, adenosine deaminase is associated with the external side of the plasma membrane. In platelets, the enzyme is associated with the external side of the plasma membrane, which is known to extend through the canalicular system of these cells. These results confirm our previous findings, based on biochemical studies, concerning the attachment of the enzyme to cell membranes.

Volume 38, Issue 5, pp. 653-658, 05/01/1990
Copyright © 1990 by The Histochemical Society


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The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1990