|
|
|
|
 |
|||
|
|||
Morphological changes induced by the calcium ionophore A23187 in rat basophilic leukemia (2H3) cells
N Sahara, RP Siraganian and C Oliver
Laboratory of Immunology, National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland 20892.
RBL-2H3 cells have been widely used to study histamine release in vitro. It was previously shown that these cells undergo striking morphological changes after IgE-mediated secretion. The present study was undertaken to examine if the morphological changes were dependent on activation of the Fc epsilon receptor. Therefore, the cells were stimulated to release histamine by two different mechanisms: activation of the Fc epsilon receptor by antigen and treatment with the calcium ionophore A23187. Cell surface and cytoskeletal changes were examined by fluorescence microscopy and scanning electron microscopy after either IgE- or ionophore-mediated histamine release. After exposure of the cells to either secretagogue, the cells spread over the surface of the culture dish and underwent rearrangement of the cytoskeleton. In addition, scanning electron microscopy revealed that deep ruffles developed on the surface of the cells undergoing IgE-mediated release. The surface changes were not as pronounced with the ionophore. The distribution of the cytoskeletal elements was examined by immunofluorescence using FITC-phalloidin and antibodies against vimentin and tubulin. In unstimulated cells actin was localized at the cell periphery, just under the plasma membrane. In the stimulated cells it was associated with the cell periphery and concentrated in the surface ruffles. As the stimulated cells spread, intermediate filaments and microtubules became distributed throughout the cell body, but there was no obvious association with the membrane ruffles. These morphological changes were dependent on the presence of extracellular calcium and on the concentration of ionophore or antigen, and were also correlated with the amount of histamine released. Additionally, IgE- mediated stimulation led to increased uptake of the soluble-phase tracer Lucifer yellow, whereas stimulation with the ionophore A23187 showed no increase in Lucifer yellow internalization. Ionophore A23187 produced changes similar but not identical to those seen in the RBL-2H3 cells after IgE-mediated histamine release. The differences may be owing to the involvement of the Fc epsilon receptor in IgE-mediated secretion.
Volume 38,
Issue 7,
pp. 975-983,
07/01/1990
Copyright © 1990 by The Histochemical Society
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  J. Holst, A. T.R. Sim, and R. I. Ludowyke Protein Phosphatases 1 and 2A Transiently Associate with Myosin during the Peak Rate of Secretion from Mast Cells Mol. Biol. Cell, March 1, 2002; 13(3): 1083 - 1098. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Draberova, E. Draberova, Z. Surviladze, P. Draber, and P. Draber Protein tyrosine kinase p53/p56lyn forms complexes with {gamma}-tubulin in rat basophilic leukemia cells Int. Immunol., November 1, 1999; 11(11): 1829 - 1839. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Glover, T. Bayburt, M. Jonas, E. Chi, and M. H. Gelb Translocation of the 85-kDa Phospholipase A[IMAGE] from Cytosol to the Nuclear Envelope in Rat Basophilic Leukemia Cells Stimulated with Calcium Ionophore or IgE/Antigen J. Biol. Chem., June 23, 1995; 270(25): 15359 - 15367. [Abstract] [Full Text] [PDF]
|
|
| Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact |