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Commercial preparations of colloidal gold-antibody complexes frequently contain free active antibody

NR Kramarcy and R Sealock

Department of Physiology, University of North Carolina, Chapel Hill 27599.

Using a simple fluorescence test, we show that commercially prepared colloidal gold complexes with goat second antibodies often contain free active antibody. Because such antibodies will compete with antibody- colloidal gold particles for antigen binding sites, labeling intensity at the ultrastructural level must necessarily be submaximal to an unknown degree with such preparations. A survey of five preparations suggests that the problem may be widespread. We recommend that a test of the sort described be incorporated routinely into protocols with all colloidal gold products.

Volume 39, Issue 1, pp. 37-39, 01/01/1991
Copyright © 1991 by The Histochemical Society


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