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Gold enhancement of gold-labeled probes: gold-intensified staining technique (GIST)

RE Morris, GM Ciraolo and CB Saelinger

Department of Anatomy and Cell Biology, University of Cincinnati College of Medicine, Ohio 45267-0521.

In this report we present a staining method in which gold chloride is used to enhance the size of gold colloids. We show the utility of this technique when used in conjunction with small gold colloids, i.e., 5 nm, 4 nm, and 2.6 nm. Post-embedding staining of epoxy-embedded, gold- labeled mouse LM fibroblasts showed that staining with 0.1% gold chloride facilitated the visualization of the smallest gold colloids.

Volume 39, Issue 11, pp. 1585-1591, 11/01/1991
Copyright © 1991 by The Histochemical Society


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