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Some monoclonal antibody reagents (C219 and JSB-1) to P-glycoprotein contain antibodies to blood group A carbohydrate determinants: a problem of quality control for immunohistochemical analysis [published erratum appears in J Histochem Cytochem 1992 Feb;40(2):318]

CL Finstad, BW Yin, CM Gordon, MG Federici, S Welt and KO Lloyd

Memorial Sloan-Kettering Cancer Center, New York, New York 10021.

Monoclonal antibodies (MAb) C219 and JSB-1 have been used extensively in the analysis of P-glycoprotein expression in normal and malignant tissues. This study demonstrates that some commercial lots of these MAb, even those supplied as purified immunoglobulins, contain contaminating anti-A blood group antibodies. In both sources of reagent, the antibody was specific for a particular A structure, known as repetitive or Type 3 A. These observations may account for earlier studies showing polymorphic variation in P-glycoprotein expression in epithelial tissues and an apparent correlation with the A blood type of the donor. Such reactivity can be eliminated by absorption of anti-P- glycoprotein reagents with A erythrocytes. These data re-emphasize the importance of evaluating MAb samples for unsuspected contaminating antibodies.

Volume 39, Issue 12, pp. 1603-1610, 12/01/1991
Copyright © 1991 by The Histochemical Society


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F. J. Hoedemaeker, T. Signorelli, K. Johns, D. A. Kuntz, and D. R. Rose
A Single Chain Fv Fragment of P-glycoprotein-specific Monoclonal Antibody C219. DESIGN, EXPRESSION, AND CRYSTAL STRUCTURE AT 2.4 A RESOLUTION
J. Biol. Chem., November 21, 1997; 272(47): 29784 - 29789.
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