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Image cytometry of progesterone receptor expression during the cell cycle in the MCF-7 cell line

S Cassanelli, P Guillaud, J Louis and D Seigneurin

Equipe de Cytologie Quantitative, Laboratoire d'Histologie, Genetique et Biologie de la Reproduction, Faculte de Medecine de Grenoble, La Tronche, France.

Progesterone receptors (PR) appear to be distributed in a heterogeneous way in mammary tumor cells. The study presented here was designed to examine if heterogeneity of PR expression is cell-cycle dependent. Immunofluorescence techniques were used to label PR on the MCF-7 human breast cancer cell line and image cytometry was used to analyze the PR expression during G0 (Ki-67 antigen-negative cells), G1, S, and G2/M cell-cycle phases. A second PR, BrdU, and DNA analysis was performed to study PR expression in the S-phase (BrdU-positive cells). Our results show that PR synthesis occurs preferentially during the G0-G1 transition and that PR levels are constant during the G1-G2 transition. The PR expression appears to be cell-cycle related and may therefore explain the heterogeneity of PR expression. However, the possibility that PR heterogeneity may be linked to the existence of PR-negative subclones cannot be ruled out.

Volume 39, Issue 12, pp. 1713-1718, 12/01/1991
Copyright © 1991 by The Histochemical Society


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