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Non-radioactive detection of nerve growth factor receptor (NGFR) mRNA in rat brain using in situ hybridization histochemistry
JE Springer, E Robbins, BJ Gwag, ME Lewis and F Baldino
Department of Neurology, Hahnemann University School of Medicine, Philadelphia, Pennsylvania 19102-1192.
Radioactively labeled RNA probes in conjunction with in situ hybridization histochemistry have become a useful method for studying gene expression in the central nervous system. We used digoxigenin- labeled uridine triphosphate to synthesize cRNA probes for localization of nerve growth factor receptor (NGFR) mRNA in the rat basal forebrain. Detection of cells containing digoxigenin-labeled NGFR mRNA was accomplished using a digoxigenin antibody conjugated with alkaline phosphatase. NGFR mRNA-positive cells were distributed in three major cell groups in the basal forebrain: the medial septal nucleus, vertical and horizontal limbs of the diagonal band of Broca, and nucleus basalis. This technique provides a rapid and sensitive method for high- resolution detection of mRNA species in the central nervous system, as well as the potential for co-localization of two different mRNA species within individual cells.
Volume 39,
Issue 2,
pp. 231-234,
02/01/1991
Copyright © 1991 by The Histochemical Society
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