|
|
|
|
 |
|||
|
|||
Simultaneous immunocytochemical visualization of bromodeoxyuridine and neural tissue antigens
E Soriano and JA Del Rio
Unidad de Biologia Celular, Facultad de Biologia, Universidad de Barcelona, Spain.
5'-Bromodeoxyuridine (BrdU) is a thymidine analogue which can be detected by monoclonal antibodies (MAb). We have developed a method for the simultaneous visualization of BrdU and a wide range of neural antigens in paraformaldehyde-fixed brain sections. Pregnant mice were injected intraperitoneally with a single pulse of BrdU. Young adult offspring were processed for immunocytochemistry following a double immunoperoxidase sequence. BrdU was detected using diaminobenzidine (DAB) intensified with nickel ammonium sulfate and neural antigen- containing elements were visualized with DAB alone. BrdU-positive nuclei and tissue antigen-immunoreactive cells were easily differentiated. Furthermore, double-labeled cells characterized by the presence of a black immunoreactive nucleus surrounded by a brown immunopositive cytoplasm were unambiguously recognized. Satisfactory results were obtained using either MAb or polyclonal antibodies against a variety of cell antigens, including neuropeptides, CA++ binding proteins, and cytoskeletal components of the glial cells. The method reported here permits analysis of the neurogenesis and proliferation of subsets of neurons and glial cells, identified by immunocytochemical markers.
Volume 39,
Issue 3,
pp. 255-263,
03/01/1991
Copyright © 1991 by The Histochemical Society
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  X. Fontana, J. Nacher, E. Soriano, and J. A. del Rio Cell Proliferation in the Adult Hippocampal Formation of Rodents and its Modulation by Entorhinal and Fimbria-Fornix Afferents Cereb Cortex, March 1, 2006; 16(3): 301 - 312. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Battaglia, S. Pagliardini, L. Saglietti, F. Cattabeni, M. Di Luca, S. Bassanini, and V. Setola Neurogenesis in Cerebral Heterotopia Induced in Rats by Prenatal Methylazoxymethanol Treatment Cereb Cortex, July 1, 2003; 13(7): 736 - 748. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. K. McCabe, D. C. Silveira, M. R. Cilio, B. H. Cha, X. Liu, Y. Sogawa, and G. L. Holmes Reduced Neurogenesis after Neonatal Seizures J. Neurosci., March 15, 2001; 21(6): 2094 - 2103. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Super, J. A. Del Rio, A. Martinez, P. Perez-Sust, and E. Soriano Disruption of Neuronal Migration and Radial Glia in the Developing Cerebral Cortex Following Ablation of Cajal-Retzius Cells Cereb Cortex, June 1, 2000; 10(6): 602 - 613. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Goldowitz, K. M. Hamre, S. A. Przyborski, and S. L. Ackerman Granule Cells and Cerebellar Boundaries: Analysis of Unc5h3 Mutant Chimeras J. Neurosci., June 1, 2000; 20(11): 4129 - 4137. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Iacopetti, M. Michelini, I. Stuckmann, B. Oback, E. Aaku-Saraste, and W. B. Huttner Expression of the antiproliferative gene TIS21 at the onset of neurogenesis identifies single neuroepithelial cells that switch from proliferative to neuron-generating division PNAS, April 13, 1999; 96(8): 4639 - 4644. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Super, A. Martinez, J. A. Del Rio, and E. Soriano Involvement of Distinct Pioneer Neurons in the Formation of Layer-Specific Connections in the Hippocampus J. Neurosci., June 15, 1998; 18(12): 4616 - 4626. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Miyata, K. Nakajima, K. Mikoshiba, and M. Ogawa Regulation of Purkinje Cell Alignment by Reelin as Revealed with CR-50 Antibody J. Neurosci., May 15, 1997; 17(10): 3599 - 3609. [Abstract] [Full Text] [PDF]
|
|
| Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact |