Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Pekki, A. K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pekki, A. K.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Different immunoelectron microscopic locations of progesterone receptor and HSP90 in chick oviduct epithelial cells

AK Pekki

Department of Biomedical Sciences, University of Tampere, Finland.

The intracellular locations of the components of the heterooligomeric progesterone receptor (PR), heat-shock protein (hsp90), and the ligand- binding component were studied by immunoelectron microscopy in the chick oviduct, using immunogold double labeling and peroxidase techniques with monoclonal antibodies (MAb) against hsp90 (7D alpha and 4F3) and against PR (PR6 and PR13). PR was located in the nuclei of epithelial cells independently of the presence or absence of ligand. Cells with apically located nuclei were often PR negative. Ten minutes after progesterone administration no apparent change was seen in PR immunoreactivity, but chromatin underwent extensive rearrangement and PR was seen at the margins of the hetero- and euchromatin. The nucleoli did not contain PR. Hsp90 was located in the cytoplasm as aggregates, often inside small vesicles. In the apical part of the cell, hsp90 was located at the Golgi complex. The nuclei contained no detectable amounts of hsp90 except for that in the nucleoli. Ten minutes after progesterone administration the location or immunoreactivity of hsp90 did not alter. Thus, there seems to be a clear difference in the intracellular distribution of PR and hsp90. The epithelium also exhibited some cells with high levels of hsp90 and no or low levels of PR. These results raise the question of whether PR is associated with hsp90 in intact cells.

Volume 39, Issue 8, pp. 1095-1101, 08/01/1991
Copyright © 1991 by The Histochemical Society


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Biol. Reprod.Home page
J. S. Tash, R. Chakrasali, S. R. Jakkaraj, J. Hughes, S. K. Smith, K. Hornbaker, L. L. Heckert, S. B. Ozturk, M. K. Hadden, T. G. Kinzy, et al.
Gamendazole, an Orally Active Indazole Carboxylic Acid Male Contraceptive Agent, Targets HSP90AB1 (HSP90BETA) and EEF1A1 (eEF1A), and Stimulates Il1a Transcription in Rat Sertoli Cells
Biol Reprod, June 1, 2008; 78(6): 1139 - 1152.
[Abstract] [Full Text] [PDF]



Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1991