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STUDIES IN HISTOCHEMISTRY: I. DETERMINATION OF NUCLEIC ACIDS IN MICROGRAM AMOUNTS OF TISSUE

JESSE F. SCOTT 1, ALBA P. FRACCASTORO 1, and EDGAR B. TAFT 1

1 From the Medical Laboratories of the Collis P. Huntington Memorial Hospital of Harvard University, at the Massachusetts General Hospital, Boston, Massachusetts

A method for the analysis of nucleic acids in microgram amounts of tissue has been devised. Results obtained by this method in rat liver, kidney, and spleen have been compared with results obtained on the same tissues by other methods of analysis for tissue nucleic acid. This method is not designed to replace the more conventional methods of analysis when milligram quantities of tissue are available, although it has been used successfully for that purpose.

Submitted on April 19, 1955


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