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PRESERVATION OF PHENYL-PHOSPHATASE ACTIVITY DURING PARAFFIN EMBEDDING

JÖRGEN B. DALGAARD 1

1 The Gade's Institute, Dep. of Pathology, Bergen, Norway

Model experiments with serum-gelatine pieces in which the effect of some basic histotechnical procedures on the phosphatase activity was studied, are reported. Following fixation and dehydration in alcohol, clearing in methyl-benzoate-celloidin, and embedding in paraffin at temperatures up to 53°C., the phosphomonoesterase activity was preserved, while embedding at higher temperatures caused increasing inactivation. The admixture of slight amounts of "soft paraffin" (m.p. 42°C.) to the ordinary 55° paraffin and embedding at 52-53° is advocated for phosphatase studies.

Submitted on May 2, 1955


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