Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by LANDING, B. H.
Right arrow Articles by HALL, H. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by LANDING, B. H.
Right arrow Articles by HALL, H. E.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

PHOSPHORYLATION AS A HISTOCHEMICAL PROCEDURE

BENJAMIN H. LANDING M.D.1 and HAZEL E. HALL 1

1 From the Departments of Pathology and Pediatrics of the Children's Hospital and Children's Hospital Research Foundation, Cincinnati, and the University of Cincinnati College of Medicine

(1) The use of phosphoryl chloride (phosphorus oxychloride) in pyridine as a reagent for phosphorylation of tissue components is described.

(2) Phosphorylation of many carbohydrate-containing substances is obtained by this method, including mucins, connective tissue (elastic tissue, reticulum, collagen, bone matrix), some glycolipids, glycogen and other tissue components.

(3) Certain chemical entities that can be phosphorylated probably do not contain carbohydrate, but are lipids (either unsaturated or incompletely esterified), or proteins with free sulfhydryl, hydroxyl, or amino groups.

(4) Phosphorylation reduces metachromasia of some tissue components (e.g. cartilage matrix), but enhances that of many others. The basophilia and metachromasia of phosphorylated components persist when staining is done in strongly acid solutions.

(5) The significance of direct leukofuchsin positivity of many phosphorylated substances, and of relatively red staining of such substances with alum hematoxylin, and aldehyde-fuchsin, is not certain.

Submitted on April 14, 1955
Accepted on June 24, 1955


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?





Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1956