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CHROMOSOMIN AND THE FEULGEN REACTION

FREDERICK H. KASTEN 1

1 Biological Station, Roswell Park Memorial Institute, Springville, New York

Evidence is presented, using the acidic dye acid fuchsin, that the Feulgen reaction is not based on acid-base mechanisms as suggested by a few authors. Various extraction, enzymatic and other procedures are used to show that the Feulgen reaction is specific for deoxyribonucleic acid when using either acid fuchsin or basic fuchsin in Schiff's reagent. There is no evidence here that any other nuclear component is stained in the Feulgen reaction.

Submitted on October 21, 1955


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