Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by VON BERTALANFFY, L.
Right arrow Articles by BICKIS, I.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by VON BERTALANFFY, L.
Right arrow Articles by BICKIS, I.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

IDENTIFICATION OF CYTOPLASMIC BASOPHILIA (RIBONUCLEIC ACID) BY FLUORESCENCE MICROSCOPY

LUDWIG VON BERTALANFFY 1 and IVAR BICKIS 1

1 Mt. Sinai Hospital, and University of Southern California, Los Angeles, and Research Institute, McGill University, Montreal, Canada

1. The fluorochrome, acridin orange (AO), allows identification of basophilic cytoplasmic inclusions in the supravital state.

2. The fluorescence picture of hepatic cells after treatment with AO shows the hyaloplasm faint gray-green; flake-like or granular structures in the cytoplasm, bright red; the nuclei, with chromatin structures, green; the central part of the nucleoli red, their periphery green.

3. The red-fluorescent cytoplasmic inclusions correspond to those observed with the toluidine-blue technique, and shown, by the RNase test, to consist mainly of RNA. The same is demonstrated by the changes of AO fluorescence after application of RNase to fresh or fixed tissues.

4. The fluorescence picture given by other cell elements and tissues in the rat liver is described.

5. Changes in the AO fluorescence of liver tissue with varying pH, dye concentration, staining time, action of salts, prolonged U.V. illumination, fixation, as well as the fluorescence of some biologically important substances (RNA, DNA, heparin, albumin, glycogen), treated with AO, are indicated.

6. The advantages and limitations of the method, and the theories of AO staining are discussed.

7. As compared to conventional histochemical methods, the applicability to living or supravital tissues of the fluorochrome technique decreases errors due to fixation, dehydration, embedding and related procedures, and the low concentrations applied in fluorochrome staining further safeguard against histochemical artifacts.


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Nutr.Home page
M. Maresca, R. Mahfoud, N. Garmy, and J. Fantini
The Mycotoxin Deoxynivalenol Affects Nutrient Absorption in Human Intestinal Epithelial Cells
J. Nutr., September 1, 2002; 132(9): 2723 - 2731.
[Abstract] [Full Text] [PDF]

Home page
 Arch SurgHome page
S. L. SCHEINER and R. E. DAVIS
Fluorescence Cytologic Screening: An Aid in the Diagnosis of Gastric Cancer
Arch Surg, December 1, 1962; 85(6): 948 - 954.
[Abstract] [PDF]

Home page
 ScienceHome page
L. VON BERTALANFFY, F. MASIN, and M. MASIN
Use of Acridine-Orange Fluorescence Technique in Exfoliative Cytology
Science, November 23, 1956; 124(3230): 1024 - 1025.
[PDF]



Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1956