|
|
|
|
 |
|||
|
|||
Quantitation of gold labeling and estimation of labeling efficiency with a stereological counting method
J Lucocq
Department of Anatomy, University of Berne, Switzerland.
The amount of immunolabeling over a cell compartment of an average cell was estimated by use of an adaptation of the double disector method introduced by Gundersen. The first and last sections of a stack of ultra-thin sections formed a disector in which cell number could be estimated and related to a defined reference volume to give the cell density. Another stack section, selected at random, was immunolabeled and the number of gold particles associated with unit volume reference space (gold "density") estimated in quadrats placed systematically across the section. The ratio of gold density to cell density was used to estimate the number of gold particles lying over a chosen compartment of an average cell, N(gold)/N(cell). Such estimates required neither cell volume nor section thickness measurement and were reproducible. By combining the number of gold particles per cell with estimates of the number of protein antigens per cell, the number of gold particles associated with each antigen could be found (labeling efficiency).
Volume 40,
Issue 12,
pp. 1929-1936,
12/01/1992
Copyright © 1992 by The Histochemical Society
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  T. Farmaki, M. Sanmartin, P. Jimenez, M. Paneque, C. Sanz, G. Vancanneyt, J. Leon, and J. J. Sanchez-Serrano Differential distribution of the lipoxygenase pathway enzymes within potato chloroplasts J. Exp. Bot., February 1, 2007; 58(3): 555 - 568. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Lucocq, A. Habermann, S. Watt, J. M. Backer, T. M. Mayhew, and G. Griffiths A Rapid Method for Assessing the Distribution of Gold Labeling on Thin Sections J. Histochem. Cytochem., August 1, 2004; 52(8): 991 - 1000. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Fahrenkrog, W. Hübner, A. Mandinova, N. Panté, W. Keller, and U. Aebi The Yeast Nucleoporin Nup53p Specifically Interacts with Nic96p and Is Directly Involved in Nuclear Protein Import Mol. Biol. Cell, November 1, 2000; 11(11): 3885 - 3896. [Abstract] [Full Text]
|
|
|
|
 |
|
 B. Fahrenkrog, E. C. Hurt, U. Aebi, and N. Pante Molecular Architecture of the Yeast Nuclear Pore Complex: Localization of Nsp1p Subcomplexes J. Cell Biol., November 2, 1998; 143(3): 577 - 588. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Santos-Rosa, H. Moreno, G. Simos, A. Segref, B. Fahrenkrog, N. Panté, and E. Hurt Nuclear mRNA Export Requires Complex Formation between Mex67p and Mtr2p at the Nuclear Pores Mol. Cell. Biol., November 1, 1998; 18(11): 6826 - 6838. [Abstract] [Full Text]
|
|
| Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact |