|
|
|
|
 |
|||
|
|||
Simultaneous fluorescence immunophenotyping and interphase cytogenetics: a contribution to the characterization of tumor cells [see comments]
K Weber-Matthiesen, M Winkemann, A Muller-Hermelink, B Schlegelberger and W Grote
Department of Human Genetics, Christian Albrechts University, Kiel, Federal Republic of Germany.
In immunocytochemical studies, the phenotypic evaluation of tumor cells is often complicated by accompanying normal cells, representing the original tissue or infiltrating leukocytes. This holds particularly true for tissues with a great morphological and immunophenotypical variability, such as bone marrow. A method that identifies mitotic tumor cells by chromosomal aberrations and permits the subsequent immunophenotypical analysis was a first progress, demonstrated by Teerenhovi et al. However, the results are usually hampered by the low number of analyzable mitoses. We demonstrate here a method that simultaneously combines immunophenotyping and in situ hybridization with centromere-specific probes. Using our method, numerically aberrant tumor cells can be identified by interphase cytogenetics and subsequently analyzed immunophenotypically. Since all interphase cells can be analyzed, we are not limited by the number and banding quality of analyzable mitoses.
Volume 40,
Issue 2,
pp. 171-175,
02/01/1992
Copyright © 1992 by The Histochemical Society
CiteULike 
Complore 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  M. Campos, C. Prior, F. Warleta, I. Zudaire, J. Ruiz-Mora, R. Catena, A. Calvo, and J. J. Gaforio Phenotypic and Genetic Characterization of Circulating Tumor Cells by Combining Immunomagnetic Selection and FICTION Techniques J. Histochem. Cytochem., July 1, 2008; 56(7): 667 - 675. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Bedell, S. J. Forman, K. Gaal, V. Pullarkat, L. M. Weiss, and M. L. Slovak Successful Application of a Direct Detection Slide-Based Sequential Phenotype/Genotype Assay Using Archived Bone Marrow Smears and Paraffin Embedded Tissue Sections J. Mol. Diagn., November 1, 2007; 9(5): 589 - 597. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. R. Cook, M. Hartke, J. Pettay, and R. R. Tubbs Fluorescence in Situ Hybridization Analysis of Immunoglobulin Heavy Chain Translocations in Plasma Cell Myeloma Using Intact Paraffin Sections and Simultaneous CD138 Immunofluorescence J. Mol. Diagn., September 1, 2006; 8(4): 459 - 465. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. A. Ventura, J. I. Martin-Subero, M. Jones, J. McParland, S. Gesk, D. Y. Mason, and R. Siebert FISH Analysis for the Detection of Lymphoma-Associated Chromosomal Abnormalities in Routine Paraffin-Embedded Tissue J. Mol. Diagn., May 1, 2006; 8(2): 141 - 151. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Bayes-Genis, M. Salido, F. Sole Ristol, M. Puig, V. Brossa, M. Camprecios, J. M Corominas, M{a} L. Marinoso, T. Baro, M{a} C. Vela, et al. Host cell-derived cardiomyocytes in sex-mismatch cardiac allografts Cardiovasc Res, December 1, 2002; 56(3): 404 - 410. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. I. Martin-Subero, I. Chudoba, L. Harder, S. Gesk, W. Grote, F. J. Novo, M. J. Calasanz, and R. Siebert Multicolor-FICTION: Expanding the Possibilities of Combined Morphologic, Immunophenotypic, and Genetic Single Cell Analyses Am. J. Pathol., August 1, 2002; 161(2): 413 - 420. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Terada, T. Yasutake, S. Nakamura, T. Hisamatsu, T. Nakagoe, H. Ayabe, and Y. Tagawa Evaluation of Metastatic Potential of Gastric Tumors by Staining for Proliferating Cell Nuclear Antigen and Chromosome 17 Numerical Aberrations Ann. Surg. Oncol., July 1, 2001; 8(6): 525 - 532. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Schwaller, E. Anastasiadou, D. Cain, J. Kutok, S. Wojiski, I. R. Williams, R. LaStarza, B. Crescenzi, D. W. Sternberg, P. Andreasson, et al. H4(D10S170), a gene frequently rearranged in papillary thyroid carcinoma, is fused to the platelet-derived growth factor receptor {beta} gene in atypical chronic myeloid leukemia with t(5;10)(q33;q22) Blood, June 15, 2001; 97(12): 3910 - 3918. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Franke, I. Wlodarska, B. Maes, P. Vandenberghe, J. Delabie, A. Hagemeijer, and C. De Wolf-Peeters Lymphocyte predominance Hodgkin disease is characterized by recurrent genomic imbalances Blood, March 15, 2001; 97(6): 1845 - 1853. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Baurmann, S. Nagel, T. Binder, A. Neubauer, W. Siegert, and D. Huhn Kinetics of the Graft-Versus-Leukemia Response After Donor Leukocyte Infusions for Relapsed Chronic Myeloid Leukemia After Allogeneic Bone Marrow Transplantation Blood, November 15, 1998; 92(10): 3582 - 3590. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P.G. Elmberger, J.A. Bridge, S.M. Noel, J.R. Neff, C.W. Walker, and S.L. Johansson Dedifferentiated Chondrosarcoma: A Report of a Case With Immunohistochemical, Ultrastructural, and Cytogenetic Analysis and Review of the Literature International Journal of Surgical Pathology, April 1, 1995; 2(4): 319 - 328. [Abstract] [PDF]
|
|
|
|
 |
|
 H. Junera, C Masson, G Geraud, and D Hernandez-Verdun The three-dimensional organization of ribosomal genes and the architecture of the nucleoli vary with G1, S and G2 phases J. Cell Sci., January 11, 1995; 108(11): 3427 - 3441. [Abstract] [PDF]
|
|
| Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact |