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A one-step cobalt-ferrocyanide method for histochemical demonstration of acetylcholinesterase activity in central nervous system tissue

CR Schatz, C Geula, RJ Morecraft and MM Mesulam

Division of Neuroscience and Behavioral Neurology, Harvard Medical School, Boston, Massachusetts.

We introduce a one-step histochemical method with cobalt as the precipitating agent for ferrocyanide for the light microscopic demonstration of acetylcholinesterase activity. This method was used to demonstrate acetylcholinesterase in normal cortical fibers and neurons, as well as pathological elements such as plaques and tangles. This procedure can also be easily combined with immunohistochemical methods that use diaminobenzidine as a chromogen.

Volume 40, Issue 3, pp. 431-434, 03/01/1992
Copyright © 1992 by The Histochemical Society


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A Simple Enzyme Histochemical Method for the Simultaneous Demonstration of Acetylcholinesterase and Monoamine Oxidase in Fixed-Frozen Sections
J. Histochem. Cytochem., June 1, 1997; 45(6): 895 - 902.
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