Retrograde tracing of zinc-containing neurons by selenide ions: a survey of seven selenium compoundsMK Christensen, CJ Frederickson and G Danscher Department of Neurobiology, University of Aarhus, Denmark. The autometallographic retrograde tracing of zinc-containing neurons by intracerebral injection of sodium selenite (Na2SeO3), introduced by Danscher in 1982, has recently been described in more detail. Intracerebral injections of both sodium selenide (Na2Se) and sodium selenite (Na2SeO3) have been successfully used; however, sodium selenite had a rather toxic effect on the injected tissue. In the present study, we tested seven different selenium compounds to find the most suitable compound for retrograde tracing of zinc-positive pathways. Among the tested compounds, sodium selenide (Na2Se) caused insignificant necrosis within the injection site and was easily transported retrogradely when handled anaerobically. Sodium selenide is therefore recommended as the compound of choice.
Volume 40,
Issue 4,
pp. 575-579,
04/01/1992
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G. Danscher and M. Stoltenberg Zinc-specific Autometallographic In Vivo Selenium Methods: Tracing of Zinc-enriched (ZEN) Terminals, ZEN Pathways, and Pools of Zinc Ions in a Multitude of Other ZEN Cells J. Histochem. Cytochem., February 1, 2005; 53(2): 141 - 153. [Abstract] [Full Text] [PDF] |
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N. Ichinohe and K. S. Rockland Region Specific Micromodularity in the Uppermost Layers in Primate Cerebral Cortex Cereb Cortex, November 1, 2004; 14(11): 1173 - 1184. [Abstract] [Full Text] [PDF] |
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R. A. Velazquez, Y. Cai, Q. Shi, and A. A. Larson The Distribution of Zinc Selenite and Expression of Metallothionein-III mRNA in the Spinal Cord and Dorsal Root Ganglia of the Rat Suggest a Role for Zinc in Sensory Transmission J. Neurosci., March 15, 1999; 19(6): 2288 - 2300. [Abstract] [Full Text] [PDF] |
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