Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Shi, S. R.
Right arrow Articles by Tandon, A. K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Shi, S. R.
Right arrow Articles by Tandon, A. K.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

A technique for retrieving antigens in formalin-fixed, routinely acid- decalcified, celloidin-embedded human temporal bone sections for immunohistochemistry

SR Shi, C Cote, KL Kalra, CR Taylor and AK Tandon

BioGenex Laboratories, San Ramon, California 94583.

The application of immunohistochemistry to routinely decalcified, celloidin-embedded human temporal bone sections has been hampered because of antigen loss during processing of the specimens. To our knowledge, there has been no published report to date describing immunohistochemical staining of such tissues suitable for examination by light microscopy. Here we report a novel antigen retrieval technique which can be successfully used to stain a variety of antigens in routinely formalin-fixed, trichloroacetic acid-decalcified, celloidin- embedded human temporal bone sections. The new procedure reported here for decalcified human temporal bone tissues simply requires immersing slides for 30 min at room temperature in an antigen retrieval solution. A total of 60 decalcified, celloidin-embedded human temporal bone tissues were tested with monoclonal antibodies (MAb) to 15 different antigens. Of these, 12 MAb showed definite positive staining, while three were negative. This technique may prove very useful in studying the expression of various antigens by immunohistochemistry in formalin- fixed, acid-decalcified, celloidin-embedded tissues.

Volume 40, Issue 6, pp. 787-792, 06/01/1992
Copyright © 1992 by The Histochemical Society


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Dent. Res.Home page
H. Arzate, L.F. Jimenez-Garcia, M.A. Alvarez-Perez, A. Landa, I. Bar-Kana, and S. Pitaru
Immunolocalization of a Human Cementoblastoma-conditioned Medium-derived Protein
J. Dent. Res., August 1, 2002; 81(8): 541 - 546.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
P. M. Almqvist, R. Mah, U. Lendahl, B. Jacobsson, and G. Hendson
Immunohistochemical Detection of Nestin in Pediatric Brain Tumors
J. Histochem. Cytochem., February 1, 2002; 50(2): 147 - 158.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
S.-R. Shi, R. J. Cote, and C. R. Taylor
Antigen Retrieval Techniques: Current Perspectives
J. Histochem. Cytochem., August 1, 2001; 49(8): 931 - 938.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
S.-R. Shi, R. J. Cote, and C. R. Taylor
Antigen Retrieval Immunohistochemistry: Past, Present, and Future
J. Histochem. Cytochem., March 1, 1997; 45(3): 327 - 344.
[Abstract] [Full Text] [PDF]



Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 1992